Melanie Coverage Tutorial experiment

You can find the Coverage Tutorial files in the folder Tutorials in the software installation directory. They come from a 2D Differential In Blot Electrophoresis (2D-DIBE) experiment aimed at assessing coverage, i.e. the percentage of immunodetection that an antibody reagent offers for a population of HCPs. An E. Coli HCP sample was pre-labeled with Cy5TM and separated on a 2D gel. Protein was transferred from the gel to a membrane. The blot was then incubated with an anti-E. Coli HCP polyclonal antibody and […]

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Melanie DIGE Tutorial experiment

You can find the DIGE Tutorial files in the folder Tutorials in the software installation directory. They come from an experiment that aimed to study protein expression changes between control and treated groups of bacterial cultures. In this study, eight sample lysates were prepared; four were derived from treated bacterial cultures and four were derived from un-treated (control) bacterial cultures. Aliquots from each sample were taken and pooled to prepare a standard sample (pooled internal standard). The samples were run on four gels. […]

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Melanie Non-DIGE Tutorial experiment

You can find the Non-DIGE Tutorial files in the folder Tutorials in the software installation directory. They come from an experiment that aimed to study protein expression changes between four conditions. Cells were grown on two different substrates (Substrate A and Substrate B) and underwent one of two treatments (Treatment 1 and Treatment 2). Three biological replicates have been run for each condition (Gel1, Gel2, Gel3). Condition Treatment Substrate A_T1 Treatment 1 Substrate A A_T2 Treatment 2 Substrate A B_T1 Treatment 1 Substrate […]

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