Differential Protein Expression Analysis

HCP Antibody Coverage Analysis

Melanie Classic / DIGE

Melanie Coverage

For scientists investigating biological processes by characterizing samples in terms of relative protein abundance and posttranslational modifications.   For scientists involved in the development or use of immunoassays aimed at monitoring Host Cell Protein (HCP) impurities in biopharmaceutical products.
Designed to detect statistically significant differences in protein expression between experimental groups, with high objectivity, sensitivity and confidence. Designed to analyze 2D-PAGE based coverage, i.e. the percentage of immunodetection that an antibody reagent offers for the total population of HCPs.
Supports images from a wide range of 2D-PAGE based methods:
• Conventional 2-DE
• 2D Differential in Gel Electrophoresis (2D-DIGE)
• Other multiplexed technologies without internal standard
Supports images from a wide range of 2D-PAGE based methods:
• Conventional 2-DE followed by 2D Western blotting
• 2D Differential In Blot Electrophoresis (2D-DIBE)
• Antibody affinity chromatography followed by 2D-DIGE
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We just launched a new software version for HCP Antibody Coverage and Differential Expression analysis

Try these exciting new features in Melanie 9.2.2!

In addition to these software-wide features, the Coverage module now includes even more functionality to minimize bias, save time and wow your team with an in-depth HCP antibody coverage analysis:

  • Coverage quadrants to ensure your antibody binds different size and charge categories of proteins
  • Coverage range to gauge the robustness of your analysis, save time and avoid the subjectivity of spot status editing
  • Presence summary to look at variations in immunodetection at the single protein level
  • Presence plot to review if coverage status edits are congruent with the presence filter thresholds

You want to learn more and see how easy, fast and reliably you can obtain results from your 2D gel and/or blot images?

Request an online demonstration

Guided step-by-step workflow

Unequaled flexibility in image visualization

Supports all common detection methods and image formats

Free Viewer functionality

MelanieTM offers significant time savings with the intuitive step-by-step workflow. Tools dedicated to each analysis stage let you feel confident that you accomplish the necessary steps and checks for the highest quality results. MelanieTM gives you full liberty over how you want to group and layout your images for viewing, and lets you choose your preferred combination of 2D and/or 3D views. No other software allows you to adjust the specific views with such ease and flexibility. MelanieTM supports any type of staining, including colorimetric, fluorescence and functional group specific stains, as well as DIGE images or western blots. Similarly, all common image formats can be analyzed, including TIFF, GEL, MEL, IMG, GSC and 1SC files. The free viewer functionality permits you to easily share your work and scientific discoveries so you can collaborate with colleagues all over the world.