Melanie Coverage Tutorial experiment

You can find the Coverage Tutorial files in the folder Tutorials in the software installation directory. They come from a 2D Differential In Blot Electrophoresis (2D-DIBE) experiment aimed at assessing coverage, i.e. the percentage of immunodetection that an antibody reagent offers for a population of HCPs. An E. Coli HCP sample was pre-labeled with Cy5TM and separated on a 2D gel. Protein was transferred from the gel to a membrane. The blot was then incubated with an anti-E. Coli HCP polyclonal antibody and […]

Read more