Introduction In the context of selecting or developing antibody reagents for HCP ELISA assays, multiple coverage analyses become essential to compare various experimental factors, including replicates, cell lines, process parameters, antigens, antibodies, and more. A critical question arises: Is there merit in analyzing image pairs from such comparisons individually, as is often the practice, or should these images be collectively analyzed in a single project? Overwhelmingly, the evidence leans toward the latter. In this discussion, we delve into why this […]

Welcome to the final chapter of our four-part series, “Image Alignment Demystified”. In our previous instalment, we shared a set of recommendations designed to enhance your alignment outcomes when utilising Melanie. Today, we’ll delve deeper into potential complications that may arise during the alignment process and illuminate key factors that require your consideration to secure optimal alignment outcomes. Navigating the density of the spot pattern The density of the spot pattern in your gel images can significantly impact alignment outcomes. […]