Melanie allows you to export a table with all spot data, also called project data. You can use the exported file for further analysis of your experiment with third party software. Learn how and what data can be exported, in what format and what export options exist.

For each spot on an image, Melanie computes the following quantification measures, based on the calibrated pixel gray values: area, background, intensity, volume, volume ratio and relative volume.

Relevant expression changes in 2D gel electrophoresis experiments are often obscured by systematic experimental variation such as differences in sample preparation, sample loading, staining/labeling or image acquisition. These sources of variation will affect different 2D gels or images to different extents, complicating the comparison of protein abundance across 2D images. The process of compensating for these variations – unrelated to the biological expression changes – is called normalization.