Melanie has a few handy keyboard shortcuts to help you save time and effort. Here’s a list of the shortcuts.

You can find the DIGE Tutorial files in the folder Tutorials in the software installation directory. They come from an experiment that aimed to study protein expression changes between control and treated groups of bacterial cultures.

You can find the Non-DIGE Tutorial files in the folder Tutorials in the software installation directory. They come from an experiment that aimed to study protein expression changes between four conditions.

For each spot on an image, Melanie computes the following quantification measures, based on the calibrated pixel gray values: area, background, intensity, volume, volume ratio and relative volume.

Melanie supports both node-locked and floating (network) licenses. This article illustrates and explains the two options, to help you choose the most appropriate license for your research environment.

Relevant expression changes in 2D gel electrophoresis experiments are often obscured by systematic experimental variation such as differences in sample preparation, sample loading, staining/labeling or image acquisition. These sources of variation will affect different 2D gels or images to different extents, complicating the comparison of protein abundance across 2D images. The process of compensating for these variations – unrelated to the biological expression changes – is called normalization.