The relevance of your image analysis results is inevitably set by the care with which you design and execute your 2D gel electrophoresis experiment. If you are new to the field, the following guides may reveal useful readings before designing and running your experiment:
DIGE is a variant of gel electrophoresis that allows for simultaneous separation of up to three samples on one gel. This methodology significantly increases statistical confidence and reliability of 2D gel electrophoresis and reduces the number of gels required per experiment.
The DIGE guide shortly explains the principles and advantages of the technique, provides references for further reading, and lists the specifics of DIGE analysis in MelanieTM.
Experimental design guide
A carefully designed experiment is key for obtaining relevant results.
The Experimental design guide provides some fundamental design notions, clarifies the design-related terminology used in MelanieTM, enumerates the designs that are specifically supported in the software in terms of statistical analysis, and explains how to proceed with the analysis of a design that is not specifically supported.
To allow successful computerized analysis, images must be generated from your 2D gels, using adequate imaging technology and settings that preserve the information contained in each gel.
The purpose of the Imaging guide is to help you produce the best possible input images, by presenting a few critical imaging notions and providing guidelines for the image acquisition process.