You probably included images when creating a new project. The Quality Control step allows you to view these images and their properties, check their quality and consistency, edit them when necessary, and validate those you want to take forward for further analysis. You can also add, remove or rename images.
Most of the functionality in this step is specifically carried out on selected images. You can select images by clicking on their thumbnail. To select multiple images, hold the Ctrl or Shift keys.
Note the difference between images and gels: gels are the physical objects for which one or several images have been acquired. In the case of DIGE and other multi-stain experiments, two or more images belong to the same gel.
To rename images or gels, click on their name above the thumbnail or in the table.
By default, the Quality control screen displays the thumbnails for all imported gel images, with below, the Quality control table. The table shows various properties of the gel images: Name, image height and width in pixels, bit depth, estimated bit depth, number of gray levels used, minimum and maximum gray levels before and after calibration, the percentage of gray levels used (calculated with respect to estimated bit depth), the percentage of the dynamic range in use (calculated with respect to estimated bit depth), the percentage saturated areas and background clipping detected, as well as the calibration formula and unit.
You can change your preferred way of viewing images and image information in the Quality control screen.
- Images/Table – Display both the image thumbnails and the quality control table.
- Images – Only display the image thumbnails, and hide the quality control table.
- Table/Histogram – Display the quality control table with the gel image and intensity histogram columns included.
Potentially problematic items, issues or values are highlighted at various levels in the Quality control screen, through icons and color codes:
- Pass – green – All looks correct, you can confidently proceed with your analysis.
- Caution – yellow – Acceptable, but be careful, Melanie detected an issues that could weaken the quality of the analysis.
- Warning – red – Anomaly identified, Melanie detected an issue that will adversely affect the quality of the analysis.
Click the More icon to display detailed information about the detected issue, how it could affect the analysis, and what you can do to solve it.
The Edit images button will take you to a dedicated Image edition mode that provides the basic tools to edit images:
(Only images that were selected in the Quality control step are opened in the Image edition mode.)
- Rotate left (90° CCW)
- Rotate right (90° CW)
- Rotate free
- Flip horizontally
- Flip vertically
- Invert gray levels
Note that editing always occurs on a copy of the imported image file; never on the original file itself.
In the Image edition mode, you will also be able to display a 3D view of your images, and to adjust their contrast. These two display options do not alter the underlying image data.
You can systematically review each gel to decide whether you want to take it forward for further analysis.
Your choice will be reflected in the Validation box at the top right of each gel. You can also tick this box to toggle between the three different states:
- The gel will be included in the analysis.
- The gel will be excluded from the analysis.
- the gel has not been validated, but will be included in the analysis by default.